Cdh2^em1(dre)Bzsh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cdh2^em1(dre)Bzsh
• Name: cadherin 2; endonuclease-mediated mutation 1, Bin Zhou
• MGI ID: MGI:6720926
• Synonyms: N-cad-LSL-Dre
• Gene: Cdh2 Location: Chr18:16721934-16942303 bp, - strand Genetic Position: Chr18, 10.1 cM
• Alliance: Cdh2^em1(dre)Bzsh page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Recombinase)
• Mutation: Insertion
• Cdh2^em1(dre)Bzsh expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cdh2 (MGI:88355)

• Mutation details: By genome editing using CRISPR-Cas9 technology, a cDNA containing loxp-stop-loxp-Dre-polyA (LSL-Dre) cassette was inserted into the translation start codon of the Cdh2 gene. To strengthen the stability of the transcripts, the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) was also included following the Dre signal sequence. (J:297057)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cdh2 (mouse) Summary of all recombinase alleles driven by Cdh2. Alliance mouse Cdh2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdh2 Mutation: 51 strains or lines available

References

• Original: J:297057 Li Y, et al., Genetic Fate Mapping of Transient Cell Fate Reveals N-Cadherin Activity and Function in Tumor Metastasis. Dev Cell. 2020 Sep 14;54(5):593-607.e5
• All: 1 reference(s)