Spats1^em1Agei
Endonuclease-mediated Allele Detail

Summary

• Symbol: Spats1^em1Agei
• Name: spermatogenesis associated, serine-rich 1; endonuclease-mediated mutation 1, Adriana Geisinger
• MGI ID: MGI:6721057
• Gene: Spats1 Location: Chr17:45754156-45785864 bp, - strand Genetic Position: Chr17, 22.44 cM, cytoband C
• Alliance: Spats1^em1Agei page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 2 bp deletion (CC) in exon 3, resulting in a frameshift at residue 90 which putatively should generate an aberrant protein of 111 amino acids instead of 269-residue protein. Western blot analysis with an antibody against the central region of the protein did not detect protein in homozygous mice. (J:307382)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spats1 Mutation: 137 strains or lines available

References

• Original: J:307382 Capoano CA, et al., SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse. PLoS One. 2021;16(5):e0251028
• All: 1 reference(s)