Ins2^{em2(dre/ERT2)Smoc}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ins2^{em2(dre/ERT2)Smoc}
• Name: insulin II; endonuclease-mediated mutation 2, Shanghai Model Organisms Center
• MGI ID: MGI:6724254
• Synonyms: Ins2^{em2(DreERT2-Wpre-polyA)Smoc}
• Gene: Ins2 Location: Chr7:142232393-142233463 bp, - strand Genetic Position: Chr7, 88.0 cM
• Alliance: Ins2^{em2(dre/ERT2)Smoc} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Ins2^{em2(dre/ERT2)Smoc} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ins2 (MGI:96573)

• Mutation details: A DreERT2-Wpre-polyA expression cassette was knocked into the initiation codon ATG site of the Ins2 gene via CRISPR/Cas mediated recombination. (J:238766, J:304636)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ins2 (mouse) Summary of all recombinase alleles driven by Ins2. Alliance mouse Ins2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ins2 Mutation: 93 strains or lines available

References

• Original: J:304636 Han X, et al., A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting. Cell Stem Cell. 2021 Feb 2
• All: 2 reference(s)