Gas2^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Gas2^{tm1c(EUCOMM)Hmgu}
• Name: growth arrest specific 2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6725132
• Gene: Gas2 Location: Chr7:51511763-51644723 bp, + strand Genetic Position: Chr7, 32.87 cM
• Alliance: Gas2^{tm1c(EUCOMM)Hmgu} page
• IMPC: Gas2 gene page

Mutation origin

• Mutant Cell Line: HEPD0681_7_F03
• Germline Transmission: Earliest citation of germline transmission: J:306028
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 51585879 of Chromosome 7 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 5 at position 51586597. The critical exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:306028)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gas2 Mutation: 18 strains or lines available

References

• Original: J:306028 Chen T, et al., Cochlear supporting cells require GAS2 for cytoskeletal architecture and hearing. Dev Cell. 2021 May 17;56(10):1526-1540.e7
• All: 2 reference(s)