Cnot4^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cnot4^{tm1d(EUCOMM)Wtsi}
• Name: CCR4-NOT transcription complex, subunit 4; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6725736
• Gene: Cnot4 Location: Chr6:34999000-35110646 bp, - strand Genetic Position: Chr6, 15.2 cM, cytoband B1
• Alliance: Cnot4^{tm1d(EUCOMM)Wtsi} page
• IMPC: Cnot4 gene page

Mutation origin

• Mutant Cell Line: EPD0682_3_G11
• Germline Transmission: Earliest citation of germline transmission: J:307645
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 35056769 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 35057701. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:307645)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnot4 Mutation: 51 strains or lines available

References

• Original: J:307645 Dai XX, et al., The CNOT4 Subunit of the CCR4-NOT Complex is Involved in mRNA Degradation, Efficient DNA Damage Repair, and XY Chromosome Crossover during Male Germ Cell Meiosis. Adv Sci (Weinh). 2021 May;8(10):2003636
• All: 1 reference(s)