Cdkn2a^{tm1(cre/ERT2)Mkt}
Targeted Allele Detail

Summary

• Symbol: Cdkn2a^{tm1(cre/ERT2)Mkt}
• Name: cyclin dependent kinase inhibitor 2A; targeted mutation 1, Makoto Nakanishi
• MGI ID: MGI:6726124
• Synonyms: p16-Cre^ERT2, p16^Ink4a-Cre^ERT2neo
• Gene: Cdkn2a Location: Chr4:89192710-89212856 bp, - strand Genetic Position: Chr4, 42.15 cM, cytoband C3-C6
• Alliance: Cdkn2a^{tm1(cre/ERT2)Mkt} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300221
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Cdkn2a^{tm1(cre/ERT2)Mkt} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cdkn2a (MGI:104738)

• Mutation details: The exon 1 alpha of the Cdkn2a gene was replaced with a translation start site, a CreERT2 expression cassette and a neo cassette. The retained neo-resistant gene markedly resulted in higher labeling efficacy at this locus presumably due to the fact that a retained neo-resistant cassette can act as a local enhancer to augment reporter expression without compromising tissue reporter fidelity. (J:300221)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cdkn2a (mouse) Summary of all recombinase alleles driven by Cdkn2a. Comparative matrix view of recombinase activities Alliance mouse Cdkn2a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdkn2a Mutation: 67 strains or lines available

References

• Original: J:300221 Omori S, et al., Generation of a p16 Reporter Mouse and Its Use to Characterize and Target p16(high) Cells In Vivo. Cell Metab. 2020 Nov 3;32(5):814-828.e6
• All: 1 reference(s)