Anks6^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Anks6^{tm1d(KOMP)Wtsi}
• Name: ankyrin repeat and sterile alpha motif domain containing 6; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6727369
• Synonyms: Anks6^tm1d
• Gene: Anks6 Location: Chr4:47015669-47057427 bp, - strand Genetic Position: Chr4, 25.88 cM
• Alliance: Anks6^{tm1d(KOMP)Wtsi} page
• IMPC: Anks6 gene page

Mutation origin

• Mutant Cell Line: EPD0109_5_E04
• Germline Transmission: Earliest citation of germline transmission: J:307271
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 47050034 of Chromosome 4 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 47050034. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene. Cre-mediated recombination deleted exon 2 and generated a knockout mouse. Western blot analysis confirmed the absence of protein in mouse embryonic fibroblasts. (J:307271)

Expression

In Mice Carrying this Mutation:	26 assay results
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Anks6 Mutation: 84 strains or lines available

Notes

Mutant cell line EPD0109_5_E04 was used to create mutant mice which demonstrated germline transmission (J:307271).

References

• Original: J:307271 Airik M, et al., Loss of Anks6 leads to YAP deficiency and liver abnormalities. Hum Mol Genet. 2020 Nov 4;29(18):3064-3080
• All: 1 reference(s)