Klhl17^em1Yph
Endonuclease-mediated Allele Detail

Summary

• Symbol: Klhl17^em1Yph
• Name: kelch-like 17; endonuclease-mediated mutation 1, Yi-Ping Hsueh
• MGI ID: MGI:6728081
• Gene: Klhl17 Location: Chr4:156313792-156319314 bp, - strand Genetic Position: Chr4, 88.63 cM
• Alliance: Klhl17^em1Yph page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology using gRNAs 5'-CTAACCTAGGCGCCACACGA (targeting the promoter region) and 5'-TAGACGTTGGAGTTCTATGG (targeting the region after exon 12) deleted the entire gene (exons 1 to 12). Western blot analysis confirmed absence of protein in homozygous neurons. (J:308069)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klhl17 Mutation: 10 strains or lines available

References

• Original: J:308069 Hu HT, et al., KLHL17/Actinfilin, a brain-specific gene associated with infantile spasms and autism, regulates dendritic spine enlargement. J Biomed Sci. 2020 Dec 1;27(1):103
• All: 2 reference(s)