Spock3^{Tg(lacZ)78-8Tyama}
Transgenic Allele Detail

Summary

• Symbol: Spock3^{Tg(lacZ)78-8Tyama}
• Name: sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3; transgene insertion 78-8, Tatsuro Yamamoto
• MGI ID: MGI:6729218
• Gene: Spock3 Location: Chr8:63404043-63810137 bp, + strand Genetic Position: Chr8, 31.97 cM
• Alliance: Spock3^{Tg(lacZ)78-8Tyama} page

Transgene origin

• Strain of Origin: C57BL/6 x C3H

Transgene description

• Transgene Type: Transgenic (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Spock3^{Tg(lacZ)78-8Tyama} involves 1 genes/genome features (Gm24236) View all
• Mutation details: Using a lacZ transgene driven by the human EEF1A1/EF1alpha promoter, a transgenic mouse line (line 78-8) was established by the transgene injection into 1-cell embryos. The genomic sequencing of the transgenic mouse revealed that both Spock3 and Anxa10 were disrupted simultaneously on chromosome 8 by the transgene insertions, resulting in double-null mutations of these genes. The transgene insertion at the Spock3 was determined to be promoterless lacZ not likely to be expressed. Spock3-deficient mice were generated from the Spock3 and Anxa10 double mutants by separating two mutant loci by repeatedly backcrossing the Spock3 and Anxa10 double-mutant founder mice on a C57BL/6 C3H hybrid genetic background to the C57BL/6 strain. Two independent animals, both on a C57BL/6 genetic background, that harbor single Spock3- mutant loci separated from the Anxa10- mutant loci (Anxa10^{Tg(EEF1A1-lacZ)78-8Tyama}) by homologous recombination were obtained. No Spock3 mRNAs were detected by in situ hybridizations in homozygous mice, confirming the null state. (J:308070)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spock3 Mutation: 34 strains or lines available

References

• Original: J:308070 Yamamoto A, et al., Structural abnormalities of corpus callosum and cortical axonal tracts accompanied by decreased anxiety-like behavior and lowered sociability in spock3- mutant mice. Dev Neurosci. 2014;36(5):381-95
• All: 1 reference(s)