Atoh1^{em1(flpo)Hlai}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Atoh1^{em1(flpo)Hlai}
• Name: atonal bHLH transcription factor 1; endonuclease-mediated mutation 1, Helen C Lai
• MGI ID: MGI:6729714
• Synonyms: Atoh1^P2A-FLPo
• Gene: Atoh1 Location: Chr6:64706109-64708229 bp, + strand Genetic Position: Chr6, 30.03 cM
• Alliance: Atoh1^{em1(flpo)Hlai} page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Atoh1^{em1(flpo)Hlai} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Atoh1 (MGI:104654)

• Mutation details: CRISPR/cas9 genome editing is used to insert a P2A, a FlpO recombinase gene after the last amino acid codon and before the stop codon of Atoh1. The cassette contained a minor mutation at the end of FLPo (the last isoleucine amino acid was changed to a serine), which could have occurred possibly because of an imprecise DNA repair event. But, this amino acid change did not affect the enzymatic function of FLPo. (J:308450)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Atoh1 (mouse) Summary of all recombinase alleles driven by Atoh1. Comparative matrix view of recombinase activities Alliance mouse Atoh1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atoh1 Mutation: 37 strains or lines available

References

• Original: J:308450 Ogujiofor OW, et al., An Atoh1 CRE Knock-In Mouse Labels Motor Neurons Involved in Fine Motor Control. eNeuro. 2021 Jan-Feb;8(1):ENEURO.0221-20.2021
• All: 3 reference(s)