Pten^tm1.1Yxy
Targeted Allele Detail

Summary

• Symbol: Pten^tm1.1Yxy
• Name: phosphatase and tensin homolog; targeted mutation 1.1, Yuxin Yin
• MGI ID: MGI:6730304
• Synonyms: ptenalpha^m, ptenalpha^mu
• Gene: Pten Location: Chr19:32734977-32803560 bp, + strand Genetic Position: Chr19, 28.14 cM
• Alliance: Pten^tm1.1Yxy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256248
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Modified isoform(s), Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Leucine codons 1 and 6 (CTG) in the alpha isoform (which is translated from an alternative CTG initiation codon 173 codons upstream of the canonical ATG codon) were replaced with glycine codons GGA (p.L1G, p.L6G). The loxP site flanked neomycin resistance gene cassette that was inserted into intron 1 was removed through subsequent cre-mediated recombination. Western blot experiments confirmed the absence of alpha isoform peptide expression from this allele in the brain, while canonical isoform expression was unaffected. (J:256248)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pten Mutation: 88 strains or lines available

References

• Original: J:256248 Wang P, et al., PTENalpha Modulates CaMKII Signaling and Controls Contextual Fear Memory and Spatial Learning. Cell Rep. 2017 Jun 20;19(12):2627-2641
• All: 5 reference(s)