Pdgfrb^{tm1.2(flpo)Leol}
Targeted Allele Detail

Summary

• Symbol: Pdgfrb^{tm1.2(flpo)Leol}
• Name: platelet derived growth factor receptor, beta polypeptide; targeted mutation 1.2, Lorin E Olson
• MGI ID: MGI:6731087
• Synonyms: bR^deltaFlp, Pdgfrb^detalFlp
• Gene: Pdgfrb Location: Chr18:61178222-61218133 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Pdgfrb^{tm1.2(flpo)Leol} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:306160
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutations: Insertion, Intragenic deletion
• Pdgfrb^{tm1.2(flpo)Leol} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pdgfrb (MGI:97531)

• Mutation details: The targeting cassette consisting of a SA.lox.PDGFRb.lox-Flpo-attB-Neo-attP cassette replaced the exons 2-4 of the endogenous Pdgfrb gene. The PDGFRb is a mouse Pdgfrb gene cDNA. PhiC31 integrase removed the attB-Neo-attP cassette. Cre-mediated recombination removed lox.PDGFRb.lox cassette from Pdgfrb^{tm1.1(flpo)Leol} to enable the expression of the flpo recombinase. This is a nock-in-nock-out null allele. (J:306160)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pdgfrb (mouse) Summary of all recombinase alleles driven by Pdgfrb. Comparative matrix view of recombinase activities Alliance mouse Pdgfrb gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfrb Mutation: 87 strains or lines available

References

• Original: J:306160 Sun C, et al., Mosaic Mutant Analysis Identifies PDGFRalpha/PDGFRbeta as Negative Regulators of Adipogenesis. Cell Stem Cell. 2020 May 7;26(5):707-721.e5
• All: 1 reference(s)