Il17a^{tm2.1(EGFP/cre/ERT2)Flv}
Targeted Allele Detail

Summary

• Symbol: Il17a^{tm2.1(EGFP/cre/ERT2)Flv}
• Name: interleukin 17A; targeted mutation 2.1, Richard A Flavell
• MGI ID: MGI:6739820
• Synonyms: IL-17A IRES-eGFP-CRE-ERT2
• Gene: Il17a Location: Chr1:20801129-20804720 bp, + strand Genetic Position: Chr1, 6.45 cM, cytoband A
• Alliance: Il17a^{tm2.1(EGFP/cre/ERT2)Flv} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224098
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Il17a^{tm2.1(EGFP/cre/ERT2)Flv} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Il17a (MGI:107364)

• Mutation details: A cassette encoding for a fusion protein consisting of the Internal Ribosome Entry Site (IRES), eGFP, Cre and human-modified Oestrogen Receptor (ERT2) (IGCE) was linked to a Frt-flanked neomycin (NEO) encoding cassette and inserted downstream of the translation stop codon in the mouse Il17a gene by targetted homologous recombination in ES cells. Germline transmitted mice were bred with germline Flippase expressing transgenic mice to remove the neomycin gene. (J:224098)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Il17a (mouse) Summary of all recombinase alleles driven by Il17a. Comparative matrix view of recombinase activities Alliance mouse Il17a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il17a Mutation: 25 strains or lines available

References

• Original: J:224098 Gagliani N, et al., Th17 cells transdifferentiate into regulatory T cells during resolution of inflammation. Nature. 2015 Jul 9;523(7559):221-5
• All: 2 reference(s)