Cpne7^{tm1.1(cre)Sleep}
Targeted Allele Detail

Summary

• Symbol: Cpne7^{tm1.1(cre)Sleep}
• Name: copine VII; targeted mutation 1.1, Yu Hayashi
• MGI ID: MGI:6739829
• Synonyms: Cpne7^Cre, Cpne7-KO
• Gene: Cpne7 Location: Chr8:123844113-123861921 bp, + strand Genetic Position: Chr8, 72.06 cM
• Alliance: Cpne7^{tm1.1(cre)Sleep} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:291955
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutation: Insertion
• Cpne7^{tm1.1(cre)Sleep} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cpne7 (MGI:2142747)

• Mutation details: Via homologous recombination in ES cells, the targeting vector inserted a nuclear localization sequence(NLS)-Cre-FRT-PGK-gb2-neo-FRT cassette (Cre cassette) downstream of the Cpne7 promoter. The PGK-Neo cassette was removed by crossing mice with CAG-FLPe mice. The western blot of whole brain samples did not show the detectable level of Cpne7 protein in homozygous mice. It is a null allele. (J:291955)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cpne7 (mouse) Summary of all recombinase alleles driven by Cpne7. Comparative matrix view of recombinase activities Alliance mouse Cpne7 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpne7 Mutation: 26 strains or lines available

References

• Original: J:291955 Liu CY, et al., Copine-7 is required for REM sleep regulation following cage change or water immersion and restraint stress in mice. Neurosci Res. 2020 Apr 11
• All: 1 reference(s)