Grpr^{em1(icre/ERT2)Ygs}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Grpr^{em1(icre/ERT2)Ygs}
• Name: gastrin releasing peptide receptor; endonuclease-mediated mutation 1, Yan-Gang Sun
• MGI ID: MGI:6740210
• Synonyms: GRPR-iCreERT2
• Gene: Grpr Location: ChrX:162296900-162332673 bp, - strand Genetic Position: ChrX, 75.63 cM
• Alliance: Grpr^{em1(icre/ERT2)Ygs} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Grpr^{em1(icre/ERT2)Ygs} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Grpr (MGI:95836)

• Mutation details: Using CRISPR/Cas9 technology, a cassette comprising of the 2A-linked optimized Cre recombinase fused with the ligand-binding domain of the estrogen receptor (2A-iCreERT2) was inserted in the Exon3 and 3UTR of Grpr gene. (J:244217)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Grpr (mouse) Summary of all recombinase alleles driven by Grpr. Comparative matrix view of recombinase activities Alliance mouse Grpr gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grpr Mutation: 14 strains or lines available

References

• Original: J:244217 Mu D, et al., A central neural circuit for itch sensation. Science. 2017 Aug 18;357(6352):695-699
• All: 7 reference(s)