Nes^{em1(flpo)Awar}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Nes^{em1(flpo)Awar}
• Name: nestin; endonuclease-mediated mutation 1, Rajeshwar Awatramani
• MGI ID: MGI:6740241
• Synonyms: Nes-LSL-Flpo, PRISM
• Gene: Nes Location: Chr3:87878400-87887758 bp, + strand Genetic Position: Chr3, 38.78 cM
• Alliance: Nes^{em1(flpo)Awar} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:290856
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Recombinase, Reporter)
• Mutation: Insertion
• Nes^{em1(flpo)Awar} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nes (MGI:101784)

• Mutation details: Using the CRISPR/Cas9 technology, the Nestin locus in ES cells were targeted to insert loxP, rox, d4GFP, SV40 poly-A, rox, stop cassette, loxP, Flpo, and bGH poly-A at the start codon of the mouse Nes gene. d4GFP is flanked by rox sites and can be removed by crossing to a Dre-deleter mouse. Cre excises the d4GFP and stop cassette, allowing Flpo expression when Nestin is expressed. (J:290856)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nes (mouse) Summary of all recombinase alleles driven by Nes. Comparative matrix view of recombinase activities Alliance mouse Nes gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nes Mutation: 82 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:290856 Poulin JF, et al., PRISM: A Progenitor-Restricted Intersectional Fate Mapping Approach Redefines Forebrain Lineages. Dev Cell. 2020 Jun 22;53(6):740-753.e3
• All: 1 reference(s)