Rr27^tm1.1Mnn
Targeted Allele Detail

Summary

• Symbol: Rr27^tm1.1Mnn
• Name: regulatory region 27; targeted mutation 1.1, Jeffrey R Mann
• MGI ID: MGI:6753234
• Synonyms: (ChbetaGI)2, H19.ICR^{tm1(ChbetaGI)MdCoh}
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm1.1Mnn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:308869
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The ICR (2.4 kb BglII fragment with chromatin insulation, promoter silencing and imprinting (differential methylation) function) between the Igf2 and H19 genes was replaced with a loxP site flanked neomycin resistance gene cassette and a 2.4 kb sequence containing two copies of the chicken beta globin chromatin insulator ((ChbetaGI)2). The neo cassette was removed through subsequent cre-mediated recombination. (J:308869)

Expression

In Mice Carrying this Mutation:

42 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

References

• Original: J:308869 Szabo PE, et al., The chicken beta-globin insulator element conveys chromatin boundary activity but not imprinting at the mouse Igf2/H19 domain. Development. 2002 Feb;129(4):897-904
• All: 2 reference(s)