Tg(Gnrh1-icre/ERT2)9Jde
Transgene Detail

Summary

• Symbol: Tg(Gnrh1-icre/ERT2)9Jde
• Name: transgene insertion 9, Jan Deussing
• MGI ID: MGI:6754136
• Synonyms: GnRH-CreERt2
• Transgene: Tg(Gnrh1-icre/ERT2)9Jde Location: unknown
• Alliance: Tg(Gnrh1-icre/ERT2)9Jde page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Gnrh1-icre/ERT2)9Jde expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gnrh1 (MGI:95789)

• Mutation details: A 208kb BAC containing the mouse Gnrh1 locus (140 kb upstream and 50 kb downstream of the ATG start codon of the Gnrh1 gene) was used to construct a minigene consisting of the fusion protein of modified estrogen receptor alpha ligand-binding protein and a codon-improved Cre recombinase followed by the polyA signal of the human growth hormone gene (hgh pA) and the Frt-flanked neoR (ERt2iCre.FrtNeoRFrt) inserted into exon 2 of the gene downstream of ATG start codon. After removal of the selection marker, the modified BAC DNA was purified and was microinjected into fertilized pronuclei to generate transgenic animals. Eleven transgenic founders were identified. Nine founders transmitted the gene to the offspring. One line (line # 9) was selected, based on its specificity and inducibility of Cre expression, for experiments. (J:288904)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gnrh1 (mouse) Summary of all recombinase alleles driven by Gnrh1. Comparative matrix view of recombinase activities Alliance mouse Gnrh1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:288904 Raftogianni A, et al., Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis. Front Mol Neurosci. 2018;11:305
• All: 1 reference(s)