Tg(Amigo2-icre/ERT2)1Ehs
Transgene Detail

Summary

• Symbol: Tg(Amigo2-icre/ERT2)1Ehs
• Name: transgene insertion 1, National Institute of Environmental Health Science
• MGI ID: MGI:6754158
• Synonyms: Amigo2-icreERT2
• Transgene: Tg(Amigo2-icre/ERT2)1Ehs Location: unknown
• Alliance: Tg(Amigo2-icre/ERT2)1Ehs page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Amigo2-icre/ERT2)1Ehs expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Amigo2 (MGI:2145995)

• Mutation details: The BAC clone RP23-288P18 was modified with recombineering techniques so that an icreERT2 is driven by the Amigo2 promotor. The Amigo2 ATG was replaced with the icre ATG preceded with a perfect KOZAK sequence. At the 3 end of the icreERT2 cassette, a synthetic bovine growth hormone (BGH) polyadenylation signal was added after the STOP codon. For the selection of recombined BACs, a flipase-site flanked neomycin resistance gene was incorporated into the targeting fragment following the icreERT2 cassette. Recombined BACs without the neo marker were linearized and purified and microinjected into pronuclei of B6SJLF1 mouse oocytes. Six independent founder mice resulted. Two lines showing adult expression of icreERT2 in CA2 were established. This is line 1 of two lines. This line showed selective expression in CA2 within the hippocampus as well as expression in the fasciola cinerea and hypothalamus. (J:288826)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Amigo2 (mouse) Summary of all recombinase alleles driven by Amigo2. Comparative matrix view of recombinase activities Alliance mouse Amigo2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:288826 Alexander GM, et al., CA2 neuronal activity controls hippocampal low gamma and ripple oscillations. Elife. 2018 Nov 2;7:e38052
• All: 3 reference(s)