Pou2f3^{tm1.1(cre/ERT2)Imt}
Targeted Allele Detail

Summary

• Symbol: Pou2f3^{tm1.1(cre/ERT2)Imt}
• Name: POU domain, class 2, transcription factor 3; targeted mutation 1.1, Ichiro Matsumoto
• MGI ID: MGI:6755141
• Synonyms: Pou2f3-CreERT2-ires-EGFP
• Gene: Pou2f3 Location: Chr9:43035222-43117052 bp, - strand Genetic Position: Chr9, 24.21 cM
• Alliance: Pou2f3^{tm1.1(cre/ERT2)Imt} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:288632
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Pou2f3^{tm1.1(cre/ERT2)Imt} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pou2f3 (MGI:102565)

• Mutation details: A CreERT2-ires-EGFP followed by an FRT-flanked PGK-Neo cassette was inserted by homologous recombination in place of the initial ATG codon of the Pou2f3 gene of C57BL/6J-derived ES cells. Chimeric mice were mated with an FLP strain to remove the PGK-Neo cassette. (J:288632)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pou2f3 (mouse) Summary of all recombinase alleles driven by Pou2f3. Comparative matrix view of recombinase activities Alliance mouse Pou2f3 gene page

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pou2f3 Mutation: 34 strains or lines available

References

• Original: J:288632 McGinty JW, et al., Tuft-Cell-Derived Leukotrienes Drive Rapid Anti-helminth Immunity in the Small Intestine but Are Dispensable for Anti-protist Immunity. Immunity. 2020 Mar 17;52(3):528-541.e7
• All: 2 reference(s)