Cd209b^{tm1.1(cre,HBEGF)Bcgen}
Targeted Allele Detail

Summary

• Symbol: Cd209b^{tm1.1(cre,HBEGF)Bcgen}
• Name: CD209b antigen; targeted mutation 1.1, Biocytogen LLC
• MGI ID: MGI:6755214
• Synonyms: Cd209b^{tm1.1(cre,DTR)Bcgen}, SIGN-R1-Cre/DTR
• Gene: Cd209b Location: Chr8:3967655-3976841 bp, - strand Genetic Position: Chr8, 1.92 cM, cytoband A1.2-A1.3
• Alliance: Cd209b^{tm1.1(cre,HBEGF)Bcgen} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:288614
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Recombinase)
• Mutation: Insertion
• Cd209b^{tm1.1(cre,HBEGF)Bcgen} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HBEGF (1839)

  Hbegf

  (MGI:96070)

• Cd209b^{tm1.1(cre,HBEGF)Bcgen} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cd209b (MGI:1916415)

• Mutation details: A cDNA construct encoding an Frt-flanked neomycin resistance cassette followed by a stop cassette was introduced before a start codon, followed by cDNA encoding Cre recombinase, and the human DTR (HBEGF) was generated. The Cre recombinase and DTR coding sequences were separated by self-cleaving 2A viral sequences to allow stoichiometric translation of both individual proteins. This construct was inserted at the ATG start codon of cd209b, effectively replacing the expression of SIGN-R1. The neomycin resistance gene and stop cassette was removed by crossing to FLPO mice. (J:288614)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cd209b (mouse) Summary of all recombinase alleles driven by Cd209b. Comparative matrix view of recombinase activities Alliance mouse Cd209b gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd209b Mutation: 30 strains or lines available

References

• Original: J:288614 Pirgova G, et al., Marginal zone SIGN-R1(+) macrophages are essential for the maturation of germinal center B cells in the spleen. Proc Natl Acad Sci U S A. 2020 Jun 2;117(22):12295-12305
• All: 1 reference(s)