Oprm1^{tm2.1(EGFP/cre)Ics}
Targeted Allele Detail

Summary

• Symbol: Oprm1^{tm2.1(EGFP/cre)Ics}
• Name: opioid receptor, mu 1; targeted mutation 2.1, Mouse Clinical Institute
• MGI ID: MGI:6755589
• Synonyms: Oprm1-Cre, Oprm1^{tm5.1(EGFP/cre)Kff}
• Gene: Oprm1 Location: Chr10:6708593-6988209 bp, + strand Genetic Position: Chr10, 1.85 cM, cytoband A2
• Alliance: Oprm1^{tm2.1(EGFP/cre)Ics} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:288326
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (No functional change, Recombinase, Reporter)
• Mutation: Insertion
• Oprm1^{tm2.1(EGFP/cre)Ics} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Oprm1 (MGI:97441)

• Mutation details: A targeting vector was designed to insert a T2A-EGFP/Cre fusion protein in exon 4 in frame just upstream of the stop codon, of the opioid receptor, mu 1 (Oprm1) coding region. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:288326)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Oprm1 (mouse) Summary of all recombinase alleles driven by Oprm1. Comparative matrix view of recombinase activities Alliance mouse Oprm1 gene page

Expression

In Mice Carrying this Mutation:

13 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Oprm1 Mutation: 54 strains or lines available

References

• Original: J:288326 Bailly J, et al., Targeting Morphine-Responsive Neurons: Generation of a Knock-In Mouse Line Expressing Cre Recombinase from the Mu-Opioid Receptor Gene Locus. eNeuro. 2020 May/Jun;7(3):ENEURO.0433-19.2020
• All: 1 reference(s)