Gsx2^{em1(flpo)Bche}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gsx2^{em1(flpo)Bche}
• Name: GS homeobox 2; endonuclease-mediated mutation 1, Bin Chen
• MGI ID: MGI:6758119
• Synonyms: Gsx2^Flpo
• Gene: Gsx2 Location: Chr5:75236262-75238554 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Gsx2^{em1(flpo)Bche} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Gsx2^{em1(flpo)Bche} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gsx2 (MGI:95843)

• Mutation details: Using a CRISPR/Cas9 based strategy, a P2A-Flpo-T2A-Flpo DNA cassette was inserted between the protein-coding sequences of exon 2 and 3' UTR of the Gsx2 gene. (J:288251)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gsx2 (mouse) Summary of all recombinase alleles driven by Gsx2. Comparative matrix view of recombinase activities Alliance mouse Gsx2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gsx2 Mutation: 18 strains or lines available

References

• Original: J:288251 Zhang Y, et al., Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog. Cell Rep. 2020 Mar 31;30(13):4490-4504.e4
• All: 1 reference(s)