Tg(Mnx1-cre/ERT2)8Rbro
Transgene Detail

Summary

• Symbol: Tg(Mnx1-cre/ERT2)8Rbro
• Name: transgene insertion 8, Robert M Brownstone
• MGI ID: MGI:6758656
• Synonyms: Hb9::CreER^T2
• Transgene: Tg(Mnx1-cre/ERT2)8Rbro Location: unknown
• Alliance: Tg(Mnx1-cre/ERT2)8Rbro page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Mnx1-cre/ERT2)8Rbro expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mnx1 (MGI:109160)

• Mutation details: The bacterial artificial chromosome (BAC) clone RP24-351I23 containing the mouse Mnx1 (Hb9) gene was modified using recombineering bacterial strain to introduce the cre/ERT2-BGH polyA and Frt-Zeo-Frt selection cassette into the exon 1 replacing the Mnx1 coding region. The Frt-Zeo-Frt cassette is retained in the transgene but lacks a eukaryotic promoter. Three founder lines (4, 8, and 10) were generated. Line 10 gave low-level inducible activity and was terminated. Line 4 and Line 8 displayed similar inducible recombination activity in the desired cell populations and were maintained. Lines 4 and 8 were indistinguishable, and line 8 was used for the majority of studies described. (J:309433)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mnx1 (mouse) Summary of all recombinase alleles driven by Mnx1. Comparative matrix view of recombinase activities Alliance mouse Mnx1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:309433 Koronfel LM, et al., Elimination of glutamatergic transmission from Hb9 interneurons does not impact treadmill locomotion. Sci Rep. 2021 Aug 6;11(1):16008
• All: 1 reference(s)