Unk^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Unk^{tm1c(KOMP)Wtsi}
• Name: unkempt family zinc finger; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6761879
• Gene: Unk Location: Chr11:115921148-115952040 bp, + strand Genetic Position: Chr11, 80.91 cM
• Alliance: Unk^{tm1c(KOMP)Wtsi} page
• IMPC: Unk gene page

Mutation origin

• Mutant Cell Line: EPD0704_5_D04
• Germline Transmission: Earliest citation of germline transmission: J:309451
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 115939326 of Chromosome 11 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 3 and 4 at position 115940459. The critical exons 3 and 4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:309451)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Unk Mutation: 68 strains or lines available

References

• Original: J:309451 Vinsland E, et al., The zinc finger/RING domain protein Unkempt regulates cognitive flexibility. Sci Rep. 2021 Aug 11;11(1):16299
• All: 1 reference(s)