Tg(Mchr1-cre/ERT2)2Nbe
Transgene Detail

Summary

• Symbol: Tg(Mchr1-cre/ERT2)2Nbe
• Name: transgene insertion 2, Nicolas F Berbari
• MGI ID: MGI:6763060
• Synonyms: Mchr1-CreER
• Transgene: Tg(Mchr1-cre/ERT2)2Nbe Location: unknown
• Alliance: Tg(Mchr1-cre/ERT2)2Nbe page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Mchr1-cre/ERT2)2Nbe expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mchr1 (MGI:2180756)

• Mutation details: A cre/ERT2 expression sequence was inserted downstream of the Mchr1 promoter on the RP23-202N16 BAC via homologous recombination/BAC recombineering; this replaced nucleotides 4-41 from exon 1 and was designed to disable transcription/translation of the Mchr1 protein from the BAC Mchr1 locus. An frt-flanked kanamycin selection cassette used during homologous recombination/BAC recombineering was removed by transient FLP plasmid injection. The resulting Mchr1-cre/ERT2 BAC transgene was purified for subsequent male pronuclear injection to establish transgenic founder animals. Two lines 2 and 3 were generated with similar expression patterns. (J:266416)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mchr1 (mouse) Summary of all recombinase alleles driven by Mchr1. Comparative matrix view of recombinase activities Alliance mouse Mchr1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:266416 Engle SE, et al., A CreER mouse to study melanin concentrating hormone signaling in the developing brain. Genesis. 2018 Aug;56(8):e23217
• All: 1 reference(s)