Tg(Sprr2f-cre)2Dcas
Transgene Detail

Summary

• Symbol: Tg(Sprr2f-cre)2Dcas
• Name: transgene insertion 2, Diego H Castrillon
• MGI ID: MGI:6764667
• Synonyms: BAC-Sprr2f-Cre
• Transgene: Tg(Sprr2f-cre)2Dcas Location: unknown
• Alliance: Tg(Sprr2f-cre)2Dcas page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Sprr2f-cre)2Dcas expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sprr2f (MGI:1330349)

• Mutation details: Using a 189 kb BAC clone (RP23-39I4) containing the entire mouse Sprr2f gene, the cre and neo cassette was inserted into the ATG start codon in exon 2 of the mouse Sprr2f gene via bacterial recombineering method. The neo selection cassette was then excised by transient flippase expression, and the resulting engineered BAC was injected into mouse oocytes for the generation of transgenic founder lines. Five founders had cre insertion, the line 2 was assigned to this transgene in absence of specific line information to distinguish it from Tg(Sprr2f-cre)1Dcas generated by the same creator which showed ectopic cre activity in brain and kidney. This transgene shows specific cre activity only in the uterus and no ectopic expression was observed. (J:282812)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sprr2f (mouse) Summary of all recombinase alleles driven by Sprr2f. Comparative matrix view of recombinase activities Alliance mouse Sprr2f gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:282812 Cuevas IC, et al., Fbxw7 is a driver of uterine carcinosarcoma by promoting epithelial-mesenchymal transition. Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25880-25890
• All: 2 reference(s)