Edil3^em1Gha
Endonuclease-mediated Allele Detail

Summary

• Symbol: Edil3^em1Gha
• Name: EGF-like repeats and discoidin I-like domains 3; endonuclease-mediated mutation 1, George Hajishengallis
• MGI ID: MGI:6780026
• Synonyms: Del1^RGE
• Gene: Edil3 Location: Chr13:88969591-89471342 bp, + strand Genetic Position: Chr13, 45.3 cM, cytoband C3
• Alliance: Edil3^em1Gha page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Single point mutation
• Mutation details: Aspartic acid codon 98 (GAC) was targeted for mutation to a glutamic acid codon (GAG)(p.D98E) with an sgRNA (UAUCGAGGAGACACAUUCAUGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUU), an ssODN template (TTTCAGGTCCCTGCATCCCTAACCCATGCCATAACGGAGGAACCTGTGAGATAAGCGAAGCCTATCGAGGAGAGACGTTTATAGGCTATGTTTGTAAATGTCCTCGGGGATTTAATGGGATTCACTGTCAGCACAGTAAGTTATTT) and a crRNA (targeting TATCGAGGAGACACATTCA) using CRISPR/Cas9 technology. The mutation renders the encoded peptide incapable of interacting with integrins. (J:299690)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Edil3 Mutation: 43 strains or lines available

References

• Original: J:299690 Yuh DY, et al., The secreted protein DEL-1 activates a beta3 integrin-FAK-ERK1/2-RUNX2 pathway and promotes osteogenic differentiation and bone regeneration. J Biol Chem. 2020 May 22;295(21):7261-7273
• All: 2 reference(s)