Prtg^tm1.1Mjfn
Targeted Allele Detail

Summary

• Symbol: Prtg^tm1.1Mjfn
• Name: protogenin; targeted mutation 1.1, Ming-Ji Fann
• MGI ID: MGI:6781728
• Gene: Prtg Location: Chr9:72714556-72824589 bp, + strand Genetic Position: Chr9, 40.08 cM
• Alliance: Prtg^tm1.1Mjfn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:311848
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES-lacZ gene replaced exons 3-7 via homologous recombination. An inserted neomycin cassette following the IRES-lacZ gene was removed. The insertion of the IRES-lacZ creates a premature termination and results in a peptide containing only the first 137 amino acids. Western blot analysis confirmed that no full-length protein is expressed in homozygous mice and immunofluorescence staining further confirmed absence of protein in embryos. (J:311848)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prtg Mutation: 212 strains or lines available

References

• Original: J:311848 Wang YC, et al., Protogenin prevents premature apoptosis of rostral cephalic neural crest cells by activating the alpha5beta1-integrin. Cell Death Dis. 2013 Jun 6;4:e651
• All: 1 reference(s)