Ankfn1^{tm2.1(cre)Mnw}
Targeted Allele Detail

Summary

• Symbol: Ankfn1^{tm2.1(cre)Mnw}
• Name: ankyrin-repeat and fibronectin type III domain containing 1; targeted mutation 2.1, Mark N Wu
• MGI ID: MGI:6782844
• Synonyms: mWAKE^Cre
• Gene: Ankfn1 Location: Chr11:89280918-89668727 bp, - strand Genetic Position: Chr11, 54.34 cM
• Alliance: Ankfn1^{tm2.1(cre)Mnw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:312000
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv x C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase, Reporter)
• Mutation: Insertion
• Ankfn1^{tm2.1(cre)Mnw} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ankfn1 (MGI:2686021)

• Mutation details: Using homologous recombination in ES cells, the knock-in vector targeted exon 5 of the Ankfn1 locus and was integrated 21 bp into exon 5, replacing the remainder of the exon with a tdTomato-P2A-split Cre-Neo-WPREBGHpA cassette, which causes frameshifted nonsense mutations downstream, resulting in the loss-of-function allele. Neomycin was excised via FLP mice. (J:312000)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ankfn1 (mouse) Summary of all recombinase alleles driven by Ankfn1. Comparative matrix view of recombinase activities Alliance mouse Ankfn1 gene page

Expression

In Mice Carrying this Mutation:

38 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ankfn1 Mutation: 48 strains or lines available

References

• Original: J:312000 Bell BJ, et al., A clock-driven neural network critical for arousal. bioRxiv. 2020
• All: 2 reference(s)