Tg(Ins1-cre,-DsRed*T3)#Caoy
Transgene Detail

Summary

• Symbol: Tg(Ins1-cre,-DsRed*T3)#Caoy
• Name: transgene insertion, Yanan Cao
• MGI ID: MGI:6790389
• Synonyms: Ins1-Cre-Dsred
• Transgene: Tg(Ins1-cre,-DsRed*T3)#Caoy Location: unknown
• Alliance: Tg(Ins1-cre,-DsRed*T3)#Caoy page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Recombinase, Reporter)
• Mutation: Insertion
• Tg(Ins1-cre,-DsRed*T3)#Caoy expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ins1 (MGI:96572)

• Mutation details: The transgene consists of an 8.5-kb fragment of mouse Ins1 promoter (Ins1; nucleotides -8.5 kb to +12 bp relative to the transcriptional start site), a nuclear location signal fused to the Cre gene fragment, an internal ribosome entry site (IRES) sequence, and a Dsred.T3 cDNA fragment followed by a polyadenylation signal. Seven lines (1-7) were generated with similar expression patterns. The pound symbol (#) is used when no line is specified and/or lines are pooled. Cre expression in different organs was examined by RT-PCR with RNA extracted from pancreatic islets, brain, liver, fat, skeletal muscle, spleen, lungs, small intestine, large intestine, kidney, heart, testes, stomach, and hypothalamus. The results showed the negligible Cre expression in tissues other than islets. (J:224637)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ins1 (mouse) Summary of all recombinase alleles driven by Ins1. Comparative matrix view of recombinase activities Alliance mouse Ins1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:224637 Cheng Y, et al., Generation and Characterization of Transgenic Mice Expressing Mouse Ins1 Promoter for Pancreatic beta-Cell-Specific Gene Overexpression and Knockout. Endocrinology. 2015 Jul;156(7):2724-31
• All: 1 reference(s)