Igs2^{tm2(Smarcd3-cre/ERT2)Bbr}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm2(Smarcd3-cre/ERT2)Bbr}
• Name: intergenic site 2; targeted mutation 2, Benoit G Bruneau
• MGI ID: MGI:6790573
• Synonyms: F6CreERT2, Smarcd3-F6CreERT2
• Gene: Igs2 Location: unknown Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm2(Smarcd3-cre/ERT2)Bbr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232545
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Igs2^{tm2(Smarcd3-cre/ERT2)Bbr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Smarcd3 (MGI:1914243)

• Mutation details: Site-specific PhiC31 integrase-mediated transgenesis is used to insert H19 insulator flanked fragment of the Smarcd3-F6 fragment, including approximately 2.5 kb of the 5 end of the Smarcd3-F1 enhancer and Hsp68 minimal promoter followed by cre/ERT coding sequence and EF1 poly signal into the intergenic site 2 locus (Hipp11). This construct was microinjected, along with the PhiC31 integrase mRNA, into the pronuclei of zygotes collected from the TARGATT mice. (J:232545)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Smarcd3 (mouse) Summary of all recombinase alleles driven by Smarcd3. Comparative matrix view of recombinase activities Alliance mouse Smarcd3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 72 strains or lines available

References

• Original: J:232545 Devine WP, et al., Early patterning and specification of cardiac progenitors in gastrulating mesoderm. Elife. 2014;3
• All: 2 reference(s)