Nkx2-5^{tm1.1(cre/ERT2)Rard}
Targeted Allele Detail

Summary

• Symbol: Nkx2-5^{tm1.1(cre/ERT2)Rard}
• Name: NK2 homeobox 5; targeted mutation 1.1, Reza Ardehali
• MGI ID: MGI:6791388
• Synonyms: Nkx2-5/CreER
• Gene: Nkx2-5 Location: Chr17:27057638-27063962 bp, - strand Genetic Position: Chr17, 13.6 cM
• Alliance: Nkx2-5^{tm1.1(cre/ERT2)Rard} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243421
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Nkx2-5^{tm1.1(cre/ERT2)Rard} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nkx2-5 (MGI:97350)

• Mutation details: Using homologous recombination in ES cells an internal ribosome entry site (IRES) linked to the gene encoding a nuclear-localizing recombinase cre fused to ERT2 and a hygromycin resistance gene cassette (pgk-HYGROpA) flanked by yeast flp recombinase target (frt) sites was inserted into the 3' UTR of the endogenous locus. pgk-HYGRO-pA cassette was removed by crossing to Flp recombinase gene expressing mice. (J:243421)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nkx2-5 (mouse) Summary of all recombinase alleles driven by Nkx2-5. Comparative matrix view of recombinase activities Alliance mouse Nkx2-5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkx2-5 Mutation: 21 strains or lines available

References

• Original: J:243421 Ranjbarvaziri S, et al., Generation of Nkx2-5/CreER transgenic mice for inducible Cre expression in developing hearts. Genesis. 2017 Aug;55(8)
• All: 2 reference(s)