En1^{tm1.1(flpo)Jbb}
Targeted Allele Detail

Summary

• Symbol: En1^{tm1.1(flpo)Jbb}
• Name: engrailed 1; targeted mutation 1.1, Jay B Bikoff
• MGI ID: MGI:6810135
• Synonyms: En1-Flpo, En1::Flpo
• Gene: En1 Location: Chr1:120530246-120535719 bp, + strand Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
• Alliance: En1^{tm1.1(flpo)Jbb} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:313825
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutation: Insertion
• En1^{tm1.1(flpo)Jbb} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	En1 (MGI:95389)

• Mutation details: Flpo, a codon-optimized version of Flp recombinase was inserted into the ATG in the 1st exon of the En1 genomic locus. Positive ES cell clones were screened by Southern blot analysis and microinjected into blastocysts, and the resulting chimeric mice were crossed to C57BL/6J females. The neomycin selectable cassette was removed using Protamine::Cre mice. (J:313825)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	En1 (mouse) Summary of all recombinase alleles driven by En1. Comparative matrix view of recombinase activities Alliance mouse En1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any En1 Mutation: 34 strains or lines available

References

• Original: J:313825 Lane AR, et al., Genetic targeting of adult Renshaw cells using a Calbindin 1 destabilized Cre allele for intersection with Parvalbumin or Engrailed1. Sci Rep. 2021 Oct 6;11(1):19861
• All: 1 reference(s)