Gck^{tm2885(icre)Thor}
Targeted Allele Detail

Summary

• Symbol: Gck^{tm2885(icre)Thor}
• Name: glucokinase; targeted mutation 2885, Bernard Thorens
• MGI ID: MGI:6810750
• Synonyms: Gck^cre, Gck^{tm2885(icre)Arte}, Gck^{tm2885(T2A-iCre)Arte}
• Gene: Gck Location: Chr11:5850820-5900081 bp, - strand Genetic Position: Chr11, 3.88 cM
• Alliance: Gck^{tm2885(icre)Thor} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:313162
• Parent Cell Line: ART B6 3.6 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Gck^{tm2885(icre)Thor} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gck (MGI:1270854)

• Mutation details: Using homologous recombination in ES cells, a T2A-iCre sequence was inserted just before the Gck STOP signal in exon10, and an FRT-flanked positive selection marker puromycin was inserted in intron 8. (J:313162)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gck (mouse) Summary of all recombinase alleles driven by Gck. Comparative matrix view of recombinase activities Alliance mouse Gck gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gck Mutation: 63 strains or lines available

References

• Original: J:313162 Kessler S, et al., Glucokinase neurons of the paraventricular nucleus of the thalamus sense glucose and decrease food consumption. iScience. 2021 Oct 22;24(10):103122
• All: 3 reference(s)