Rasef^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rasef^em1Osb
• Name: RAS and EF hand domain containing; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6830921
• Gene: Rasef Location: Chr4:73632816-73709231 bp, - strand Genetic Position: Chr4, 36.06 cM
• Alliance: Rasef^em1Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 63,153 bp deletion using gRNAs AGCATGACTTCCTTGTGGCG and GCTTCAGGTTGCTGAGAGCG. (J:315008)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rasef Mutation: 55 strains or lines available

References

• Original: J:315008 Oyama Y, et al., CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice. Asian J Androl. 2021 Jul 16
• All: 1 reference(s)