Cckbr^{tm1.1(cre)Mgmj}
Targeted Allele Detail

Summary

• Symbol: Cckbr^{tm1.1(cre)Mgmj}
• Name: cholecystokinin B receptor; targeted mutation 1.1, Martin G Myers, Jr
• MGI ID: MGI:6834787
• Synonyms: Cckbr^Cre
• Gene: Cckbr Location: Chr7:105075201-105085546 bp, + strand Genetic Position: Chr7, 55.86 cM
• Alliance: Cckbr^{tm1.1(cre)Mgmj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:316161
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Cckbr^{tm1.1(cre)Mgmj} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cckbr (MGI:99479)

• Mutation details: Using homologous recombination in ES cells, a T2A sequence and a CreER(T2) fusion gene were inserted immediately downstream of the cholecystokinin B receptor locus (Cckbr) on chromosome 7. The targeting vector contained, from 5' to 3', a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping), a Cre recombinase gene, a bovine growth hormone polyA sequence, an frt site, a PGK-neo-polyA cassette, and an frt site. Founder mice were bred with Flpo-expressing mice to delete the PGK-neo-polyA cassette. (J:316161)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cckbr (mouse) Summary of all recombinase alleles driven by Cckbr. Comparative matrix view of recombinase activities Alliance mouse Cckbr gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cckbr Mutation: 24 strains or lines available

References

• Original: J:316161 Flak JN, et al., Ventromedial hypothalamic nucleus neuronal subset regulates blood glucose independently of insulin. J Clin Invest. 2020 Jun 1;130(6):2943-2952
• All: 1 reference(s)