Psmf1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Psmf1^{tm1c(EUCOMM)Hmgu}
• Name: proteasome (prosome, macropain) inhibitor subunit 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6835662
• Synonyms: PI31^fl
• Gene: Psmf1 Location: Chr2:151557982-151586114 bp, - strand Genetic Position: Chr2, 74.83 cM
• Alliance: Psmf1^{tm1c(EUCOMM)Hmgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282431
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 151575920 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 151576689. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and the neo selection cassette, leaving loxP sites flanking the critical exon 3. (J:282431)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Psmf1 Mutation: 13 strains or lines available

References

• Original: J:282431 Minis A, et al., The proteasome regulator PI31 is required for protein homeostasis, synapse maintenance, and neuronal survival in mice. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24639-24650
• All: 1 reference(s)