Cenpv^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cenpv^{tm1d(EUCOMM)Hmgu}
• Name: centromere protein V; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6838384
• Gene: Cenpv Location: Chr11:62415772-62430087 bp, - strand Genetic Position: Chr11, 38.43 cM, cytoband B2
• Alliance: Cenpv^{tm1d(EUCOMM)Hmgu} page
• IMPC: Cenpv gene page

Mutation origin

• Mutant Cell Line: HEPD0517_1_G11
• Germline Transmission: Earliest citation of germline transmission: J:314905
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 62424450 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 62425249. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:314905)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cenpv Mutation: 60 strains or lines available

References

• Original: J:314905 Nabi D, et al., CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes. Nat Commun. 2021 Nov 11;12(1):6547
• All: 1 reference(s)