Tg(Ins2-tTA,tetO-cre)1Ctlh
Transgene Detail

Summary

• Symbol: Tg(Ins2-tTA,tetO-cre)1Ctlh
• Name: transgene insertion 1, Carol T L Huang
• MGI ID: MGI:6852696
• Synonyms: RIP-tTA/tetO7-Cre
• Transgene: Tg(Ins2-tTA,tetO-cre)1Ctlh Location: unknown
• Alliance: Tg(Ins2-tTA,tetO-cre)1Ctlh page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Ins2-tTA,tetO-cre)1Ctlh expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ins2 (MGI:96573)

• Mutation details: A 3.5 Kb fragment from a plasmid carrying a rat insulin Ins2-tTA cassette and a 3.9 Kb fragment from a plasmid carrying TetO7 cloned upstream of Cre-encoding DNA were co-injected into fertilized oocytes to produce double-transgenic mice. Five lines were generated and one line showed cre activity doxycycline dependant manner, on with the absence and off with the presence of doxycycline, in islet cells. (J:244123)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ins2 (mouse) Summary of all recombinase alleles driven by Ins2. Comparative matrix view of recombinase activities Alliance mouse Ins2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:244123 Toselli C, et al., Contribution of a non-beta-cell source to beta-cell mass during pregnancy. PLoS One. 2014;9(6):e100398
• All: 1 reference(s)