Gpx4^tm5Marc
Targeted Allele Detail

Summary

• Symbol: Gpx4^tm5Marc
• Name: glutathione peroxidase 4; targeted mutation 5, Marcus Conrad
• MGI ID: MGI:6874619
• Synonyms: Gpx4^cys
• Gene: Gpx4 Location: Chr10:79883000-79892273 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Gpx4^tm5Marc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:255340
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Single point mutation
• Mutation details: Exon 3 was replaced with a modified one in which a point mutation (UGA to UGC) results in the substitution of the catalytically active selenocysteine to a cysteine. A loxP-flanked neomycin resistance cassette was inserted downstream of exon 7. GPX4-specific activity is undetectable in homozygous tissues using PCOOH as substrate. (J:255340)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpx4 Mutation: 82 strains or lines available

References

• Original: J:255340 Ingold I, et al., Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis. Cell. 2018 Jan 25;172(3):409-422.e21
• All: 3 reference(s)