Mecp2em2Gfng
Endonuclease-mediated Allele Detail
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Symbol: |
Mecp2em2Gfng |
Name: |
methyl CpG binding protein 2; endonuclease-mediated mutation 2, Guoping Feng |
MGI ID: |
MGI:6886060 |
Synonyms: |
Mecp2 huExon4 |
Gene: |
Mecp2 Location: ChrX:73070198-73129296 bp, - strand Genetic Position: ChrX, 37.63 cM
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Alliance: |
Mecp2em2Gfng page
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Allele Type: |
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Endonuclease-mediated (Humanized sequence, Inserted expressed sequence) |
Mutations: |
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Insertion, Nucleotide substitutions
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Mecp2em2Gfng expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
MECP2 (4204) |
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Human exon 4 |
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Mutation details: CRISPR/cas9 genome editing used two guides sgRNA-49229 (5'-gattgcatactttgaaaagg-3') and sgRNA-49791 (5'-ccacccttggtgagaaaagc-3') to target the start and end of the humanization region (exon 4) as well as a single strand DNA (ssDNA) donor template that spans the two cut sides. The homology arm length for the ssDNA donor is 300 nt. The insertion is comprised of a 561 bp humanized MECP2 region, which corresponds to amino acid (aa) positions 145-331 aa in the human MeCP2 locus (Note: brain enriched isoform MeCP2-E1 is 498 aa in length).
(J:101977)
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
1 reference(s) |
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