Taf1b^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Taf1b^{tm1c(EUCOMM)Hmgu}
• Name: TATA-box binding protein associated factor, RNA polymerase I, B; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6887476
• Synonyms: Taf1b^fl
• Gene: Taf1b Location: Chr12:24548580-24608570 bp, + strand Genetic Position: Chr12, 8.49 cM, cytoband A3
• Alliance: Taf1b^{tm1c(EUCOMM)Hmgu} page

Mutation origin

• Mutant Cell Lines: HEPD0596_3_G02, HEPD0596_3_H01
• Germline Transmission: Earliest citation of germline transmission: J:321558
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 24558604 of Chromosome 12 upstream of the critical exons 4 and 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 4 and 5 at position 24560225. The critical exons 4 and 5 are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene and generates a conditional ready (floxed) allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:321558)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Taf1b Mutation: 36 strains or lines available

References

• Original: J:321558 Tremblay MG, et al., Ribosomal DNA promoter recognition is determined in vivo by cooperation between UBTF1 and SL1 and is compromised in the UBTF-E210K neuroregression syndrome. PLoS Genet. 2022 Feb;18(2):e1009644
• All: 1 reference(s)