Tubb4a^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Tubb4a^{tm1c(EUCOMM)Hmgu}
• Name: tubulin, beta 4A class IVA; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6887962
• Synonyms: Tubb4a^fl
• Gene: Tubb4a Location: Chr17:57387061-57394600 bp, - strand Genetic Position: Chr17, 29.66 cM
• Alliance: Tubb4a^{tm1c(EUCOMM)Hmgu} page
• IMPC: Tubb4a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:321692
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 57392855 of Chromosome 17 upstream of the critical exons 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2 and 3 at position 57393590. The critical exons 2 and 3 are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neo cassette, creating a conditional ready (floxed) allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:321692)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tubb4a Mutation: 30 strains or lines available

References

• Original: J:321692 Fertuzinhos S, et al., A dominant tubulin mutation causes cerebellar neurodegeneration in a genetic model of tubulinopathy. Sci Adv. 2022 Feb 18;8(7):eabf7262
• All: 1 reference(s)