Gt(ROSA)26Sor^{tm1.1(CAG-YFP/DROSHA*E1147K)Gess}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-YFP/DROSHA*E1147K)Gess}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Manfred Gessler
• MGI ID: MGI:6887963
• Synonyms: DROSHA-E1147K
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-YFP/DROSHA*E1147K)Gess} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:321428
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(CAG-YFP/DROSHA*E1147K)Gess} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DROSHA (29102)

  Drosha

  (MGI:1261425)

  CAG promoter is separated from YFP/DROSHA fusion gene by floxed puro cassette, so is only expressed after cre-mediated deletion of puro. Human DROSHA has C-terminal FLAG tag sequences.

• Mutation details: Using ES cells containing the RMCE-ready Gt(ROSA)26Sor^tm1Rkuhn allele, the original cassette was exchanged with a vector containing the following elements: an attB site, the CAG promoter, a loxP site flanked puromycin resistance gene cassette, a YFP gene in-frame with a human DROSHA cDNA with a p.E1147K mutation and C-terminal FLAG tag sequences, and a second attB site. The mutated Drosha will only be expressed after cre-mediated deletion of the puro cassette. The mutation is associated with Wilms Tumor and critically affects the metal-binding Drosha needs for its cleaving activity. (J:321428)
• Inheritance: Dominant

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:321428 Kruber P, et al., Loss or oncogenic mutation of DROSHA impairs kidney development and function, but is not sufficient for Wilms tumor formation. Int J Cancer. 2019 Mar 15;144(6):1391-1400
• All: 1 reference(s)