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Gt(ROSA)26Sortm1.1(CAG-YFP/DROSHA*E1147K)Gess
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm1.1(CAG-YFP/DROSHA*E1147K)Gess
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Manfred Gessler
MGI ID: MGI:6887963
Synonyms: DROSHA-E1147K
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm1.1(CAG-YFP/DROSHA*E1147K)Gess page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:321428
Parent Cell Line:  IDG3.2 (ES Cell)
Strain of Origin:  (C57BL/6J x 129S6/SvEvTac)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Gt(ROSA)26Sortm1.1(CAG-YFP/DROSHA*E1147K)Gess expresses 1 gene
 
Mutation detailsUsing ES cells containing the RMCE-ready Gt(ROSA)26Sortm1Rkuhn allele, the original cassette was exchanged with a vector containing the following elements: an attB site, the CAG promoter, a loxP site flanked puromycin resistance gene cassette, a YFP gene in-frame with a human DROSHA cDNA with a p.E1147K mutation and C-terminal FLAG tag sequences, and a second attB site. The mutated Drosha will only be expressed after cre-mediated deletion of the puro cassette. The mutation is associated with Wilms Tumor and critically affects the metal-binding Drosha needs for its cleaving activity. (J:321428)
Inheritance:    Dominant
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 3 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  992 strains or lines available
References
Original:  J:321428 Kruber P, et al., Loss or oncogenic mutation of DROSHA impairs kidney development and function, but is not sufficient for Wilms tumor formation. Int J Cancer. 2019 Mar 15;144(6):1391-1400
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory