Tmprss2em1(TMPRSS2)Synbl
Endonuclease-mediated Allele Detail
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Symbol: |
Tmprss2em1(TMPRSS2)Synbl |
Name: |
transmembrane protease, serine 2; endonuclease-mediated mutation 1, Synbal |
MGI ID: |
MGI:7261377 |
Gene: |
Tmprss2 Location: Chr16:97365882-97412395 bp, - strand Genetic Position: Chr16, 57.53 cM, cytoband C2
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Alliance: |
Tmprss2em1(TMPRSS2)Synbl page
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Allele Type: |
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Endonuclease-mediated (Humanized sequence, Inserted expressed sequence, Null/knockout) |
Mutation: |
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Insertion
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Tmprss2em1(TMPRSS2)Synbl expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
TMPRSS2 (7113) |
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Mutation details: Cas9 and gRNA Tmprss2-crRNA_5 (protospacer sequence 5-GTGCTCAGACTGATACCCG-3) are used to introduce the following sequences at codon 19 in exon 3: GGSG linker, P2A, human TMPRSS2 cDNA coding sequence with SV40 intron inserted for enhanced expression, 1837 bp human TMPRSS2 3UTR, 112 bp human intergenic sequence immediately 3 of TMPRSS2 polyadenylation region , a cassette comprised of a mouse U6 promoter-driven Tmprss2-gRNA5 guide RNA with 2xPolIII terminator, all in reverse orientation relative to Tmprss2 gene, splice acceptor and SV40 polyadenylation sequence followed by a Flp Recombinase Target (FRT) site and 3-frame ATG short open reading frames. This cassette is designed to trap and truncate any mouse Tmprss2 isoforms that may splice past the hTMPRSS2 cassette. The FRT site allows Flp-mediated collapse of targeted tandem integration events if required.
(J:101977)
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
1 reference(s) |
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