Fcgrtem1(FCGRT)Synbl
Endonuclease-mediated Allele Detail
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Symbol: |
Fcgrtem1(FCGRT)Synbl |
Name: |
Fc fragment of IgG receptor and transporter; endonuclease-mediated mutation 1, Synbal |
MGI ID: |
MGI:7261380 |
Gene: |
Fcgrt Location: Chr7:44742417-44753246 bp, - strand Genetic Position: Chr7, 29.12 cM
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Alliance: |
Fcgrtem1(FCGRT)Synbl page
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Allele Type: |
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Endonuclease-mediated (Humanized sequence, Inserted expressed sequence, Null/knockout) |
Mutation: |
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Insertion
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Fcgrtem1(FCGRT)Synbl expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
FCGRT (2217) |
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Mutation details: Cas9 and gRNA Fcgrt-g91T (protospacer sequence 5- GGGTCACCCTGTCGGAATG-3) are used to introduce the following sequence at the start codon in exon 2: Kozak sequence and human FCGRT cDNA cassette inserted at the cleavage site, Sv40 intron, a 214 bp native hFCGRT 3UTR (3UTR), a 152 bp native human sequence 3 of the mapped 3UTR, and a mouse U6 promoter-driven guide RNA scaffold with 2xPolIII terminator (all in reverse orientation immediately 3 of the hFCGRT cassette), a splice-trap element with splice acceptor and SV40 polyadenylation sequence followed by 3-frame ATG short open reading frames. This active genetics cassette is designed to trap and truncate any mouse Fcgrt isoforms that may splice past the hFCGRT cassette. A Flp Recombinase Target (FRT) site is inserted to allow Flp-mediated collapse of targeted tandem integration events (if needed).
(J:101977)
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
2 reference(s) |
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