Hlf^{em1(cre/ERT2)Smoc}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hlf^{em1(cre/ERT2)Smoc}
• Name: hepatic leukemia factor; endonuclease-mediated mutation 1, Shanghai Model Organisms Center
• MGI ID: MGI:7262729
• Synonyms: Hlf^CreER
• Gene: Hlf Location: Chr11:90227362-90281721 bp, - strand Genetic Position: Chr11, 55.03 cM
• Alliance: Hlf^{em1(cre/ERT2)Smoc} page

Mutation origin

• Strain of Origin: C57BL/6JSmoc

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Hlf^{em1(cre/ERT2)Smoc} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Hlf (MGI:96108)

• Mutation details: CRISPR/Cas9 genome editing technology was used to insert a 2A-Cre and rox flanked ERT2 expression cassette into the exon 4 and 3' UTR of the Hlf gene. (J:312489)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hlf (mouse) Summary of all recombinase alleles driven by Hlf. Comparative matrix view of recombinase activities Alliance mouse Hlf gene page

Expression

In Mice Carrying this Mutation:

18 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hlf Mutation: 17 strains or lines available

References

• Original: J:312489 Tang W, et al., Hlf Expression Marks Early Emergence of Hematopoietic Stem Cell Precursors With Adult Repopulating Potential and Fate. Front Cell Dev Biol. 2021;9:728057
• All: 1 reference(s)