Cant1^tm1.2Aros
Targeted Allele Detail

Summary

• Symbol: Cant1^tm1.2Aros
• Name: calcium activated nucleotidase 1; targeted mutation 1.2, Antonio Rossi
• MGI ID: MGI:7264674
• Synonyms: Cant1^-
• Gene: Cant1 Location: Chr11:118297115-118309912 bp, - strand Genetic Position: Chr11, 83.15 cM
• Alliance: Cant1^tm1.2Aros page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282272
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J x 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A G to A transition at position 905 (c.G905A) resulting in an arginine to histidine substitution at amino acid 302 (p.R302H) was introduced in exon 4. In addition, an FRT-flanked neomycin selection cassette and loxP site were inserted upstream of exon 3 and a second loxP site was inserted downstream of mutated exon 4. The selection cassette was removed via flp-mediated recombination and cre-mediated recombination deleted exons 3 and 4. Real-time PCR confirmed absence of mRNA in skin. (J:282272)

Disease models

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cant1 Mutation: 28 strains or lines available

References

• Original: J:282272 Paganini C, et al., Calcium activated nucleotidase 1 (CANT1) is critical for glycosaminoglycan biosynthesis in cartilage and endochondral ossification. Matrix Biol. 2019 Aug;81:70-90
• All: 1 reference(s)