Mmrn1^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Mmrn1^{tm1d(EUCOMM)Hmgu}
• Name: multimerin 1; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7266885
• Synonyms: Mmrn1^-
• Gene: Mmrn1 Location: Chr6:60921301-60966362 bp, + strand Genetic Position: Chr6, 29.22 cM, cytoband C1
• Alliance: Mmrn1^{tm1d(EUCOMM)Hmgu} page
• IMPC: Mmrn1 gene page

Mutation origin

• Mutant Cell Line: HEPD0617_6_G03
• Germline Transmission: Earliest citation of germline transmission: J:307643
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 60937222 of Chromosome 6 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 60938096. Exon 3 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon. Western blot analysis of platelet lysates confirmed the complete absence of protein in platelets from homozygous mutant mice. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:307643)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mmrn1 Mutation: 63 strains or lines available

References

• Original: J:307643 Leatherdale A, et al., Multimerin 1 supports platelet function in vivo and binds to specific GPAGPOGPX motifs in fibrillar collagens that enhance platelet adhesion. J Thromb Haemost. 2021 Feb;19(2):547-561
• All: 1 reference(s)